Abstract
Intervening intron-spacer between sense and antisense sequences forms the most effective dsRNA structures in target gene disruption by RNAi. Previous our research showed that RNAi vector WRI-A with Wx promoter, the 5'UTR sense and antisense Wx sequences, intron-spacer could lead to complete suppression of Wx expression in endosperm, but not in pollen. By contrast, WRI-B carries the substitution mutation at 5'splice site of the intron-spacer could lead to lower degree of Wx suppression endosperm. Further studies examined the effects of various vectors on RNAi as follows; 1) comparison of efficiency of the vectors with another mutation at splice site of intron-spacer, 2) the influence of the dsRNA with different trigger regions on tissue specific suppression of Wx, 3) effect of trigger length of dsRNA on degree of Wx suppression. And results from these experiments will be discussed.
