Abstract
Previously, we reported binary vectors compatible for Gateway Cloning Technology. They were used for construction of reporter/tag fusion genes. In this paper, we report the Dual Site Gateway Binary Vector System for cloning of two genes. With this system, reporter / tag sequence is independently fused to two genes. This system is very useful for plant research, because the biochemical and cellular analyses of interaction between two genes interested are very important.
In this paper, we report the vector containing new recombination sequences attR3- attR4 in addition to attR1-attR2. Using this vector, two genes were able to be cloned in one vector by LR reaction. We also report application of this system.
