Abstract
We isolated 6 different cDNAs (VHA-c1 to VHA-c6) coding for the proteolipid subunit of Acetabularia acetabulum (A. a) V-ATPase. By heterologous expression in yeast, the 6 translation products were revealed to function as Vma3p, but not as Vma11p. By using specific antibodies, the two isoforms (VHA-c2/VHA-c4) were detected in a microsomal fraction prepared from stalks of A. a cells before cap formation (Adult). In whorl of hairs of Adult cells, VHA-c2 was detected. In caps of A. a cells after cap formation (Reproductive), VHA-c1, -c2 and -c4 were detected. V-PPase was detected in stalks, whorl of hairs and caps of Reproductive cells. In the case of stalks and whorl of hairs of Reproductive cells, similar results were obtained as Adult cells. VHA-c1, -c2 and -c4 were immunohistochemically revealed to be localized in vacuolar membrane of caps.
