Abstract
AP2/EREBP family consists one of the largest groups of transcriptional factors in plants. Previously we showed that AtEBP, one of AP2/EREBP proteins, functioned as a transcriptional factor and plant cells over-expressing AtEBP conferred stress resistances.
Tobacco plants over-expressing AtEBP demonstrated the resistance to Bax, mammalian apoptosis factor, -induced cell death. Whether AtEBP expression affects a process of generating reactive oxygen species (ROS) caused by Bax expression, we performed NBT and DAB staining using leaves expressing both Bax and, Bax and AtEBP. ROS accumulation by Bax was observed in organelle despite AtEBP expression, suggesting that AtEBP expression did not affect a process of generating ROS. Arabidopsis plants over-expressing AtEBP did not show significant differences to GST, GR activities and the resistance for H2O2 and MV treatments. We also found that plant defensin PDF1.2 expression was regulated by AtEBP expression level. The biological function of AtEBP in stress response will be discussed.
