Abstract
Chlamydomonas, a model organism of green algae, has been used for research such as photosynthesis and flagellar, although the regulatory mechanism of cell division and cell growth, have been obscured.
On 2002nd annual meeting, we have reported the isolation and analysis of Chlamydomonas MAP kinase homologues, CrMPK1 and CrMPK2, although the stimuli for activation of each kinase has been unclear. For detection of each kinase separately, we had prepared anti-CrMPK1-specific and anti-CrMPK2-specific antibodies, respectively. With these antibodies, we had screened the conditions and stimuli by which each kinase had been phosphorylated. As a result, CrMPK1 kinase was transiently phosphorylated just before the initiation of cell division on synchronized cell cultivation. This result suggested that CrMPK1 kinase is related to the regulation of cell division.
