Abstract
Genomic information of tomato is increasing with the progress of tomato genome project. By contrast, metabolic information of tomato is not sufficient to systematically link it to genomic information. Although the metabolic profiling of primary metabolites from tomato fruit has been reported, there has been no report on comprehensive analysis of secondary metabolites. In this study, we analyzed metabolites of tomato (cv. Micro-Tom) fruit comprehensively using LC-FT-MS. LC-FT-MS facilitates measurement of m/z value at sub milli-mass unit. We collected information of each compound, such as retention time, accurate molecular weight, MS/MS data, and UV absorption. More than 500 compounds from tomato fruits were detected, and their chemical information was obtained by comparing our data with open metabolite databases (KNApSAcK, DNP). We also found that the composition of metabolites was different in fruit tissues and maturing stages. Furthermore, many unknown metabolites were detected from tomato fruit.
