Abstract
In this study, we focus on obtaining the profile of secretory proteins of rice root, and compare the change in the profile among different nutrient stress, to study secretory proteins comprehensively.
After germination, rice were grown in three different culture condition; control, -P and -N for two weeks. Solution was renewed everyday, and the protein sample was collected from the culture solution of the last day. Sample solutions were concentrated. After 2D gel electrophoresis, protein profile was appeared. Many proteins in control treatment indicate that rice secretes proteins constitutively from root even under normal condition. And it was also found that different protein profile was found by -P and -N treatments. Major spots and those spots behaved specifically by the treatments were picked, and the identification of them by MALDI-TOF-MS is being carried forward.
