Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 47
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Pathway analysis by proteome and transcriptome of rice root against low phosphorus condition
Akira Saito*Takuro ShinanoShunji TokutakeJun WasakiMitsuru Osaki
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Pages 009

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Abstract
We used the same sample for simultaneous transcriptome and proteome analysis, and analyzed using KaPPA view. From the transcriptome analysis, when sufficient phosphorus supplied treatment was put as a control, it was observed increased expression of 1) glycolysis related genes, 2) sufolipid and glycolipid synthesizing related genes, stable in 3) TCA cycle composing enzymes genes, and 4) anion channel coding gene, and decreased in 5) amino acids synthesis related genes under phosphorus limited condition (-P). When phosphorus was supplied to -P on one day before the sampling, a decrease in the gene expression was observed in glycolysis related genes. On the other hand, from proteome analysis, though similar tendency was observed in glycolysis related enzymes, the response was reverse in amino acids synthesis related enzymes. And significant increase in anion channel was observed under -P. Thus the fluctuation pattern by phosphorus status was not consistent between transcriptome and proteome.
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© 2006 by The Japanese Society of Plant Physiologists
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