Abstract
Tension wood is a reaction wood of angiosperm woody plants. Reaction wood causes the stem to bend by its mechanical action. Tension wood generates much higher tensile growth stress than normal wood. However, the mechanisms how the tissue switches normal to tension wood, or how it generates tensile stress in tension wood is still unknown. Buffer insoluble proteins from tension wood and normal wood were compared on 2-D PAGE. The signals of which sizes were increased in tension side were cut off from the gel, digested by trypsin, then analyzed by LC/MS/MS. The proteins were searched on database with the mass spectrum patterns that obtained by LC/MS/MS. Many enzymes were annotated but nothing was found as a significant protein which was able to explain neither differentiation of tension wood nor generation of tensile stress.
