Abstract
We have reported the adapted mechanism for boron deficiency by using poplar cells (1/20-B) grown in low boron concentration medium (5 μM). In these cells, pectin methylesterase (PME) activity was higher than that of non-resistant cells (1/1-B). This result indicates that the increase of activity plays a part of the mechanism in tolerance to boron deficiency.
In this study, I report the expression of PME gene in both cell lines during a culture period. The expression of PME gene in 1/1-B was observed only in 1-9 day after subculture. However, that of 1/20-B was recorded through the 14-days of culture period. Because the boron deficiency affects in cell elongation growth rather than cell division, and the later half of culture period was cell elongation phase in this cell line, it was indicated that the expression of PME gene in cell elongation phase took part in the tolerance to boron deficiency.
