Abstract
The comparison between gene expression profiles revealed that there were 45 RLKs expressed in pollen and/or pollen tube of Arabidopsis thaliana. Single gene T-DNA insertion lines did not show any strong phenotypes and double disruptions in sister genes seemed to be required for the further analyses. We made 10 sets of double gene disruptant lines, which covered 20 genes.
We found one line, whose pollen germination rate in vitro reduced to almost 0% using the wild type as 100% control. Although the segregation rate in self-fertilization looked normal, the seed numbers, especially in first 10 siliques, were reduced to half of wild type in their median. There might be interfered pollen germination and elongation in smaller number of pollen attached on the papilla. We also found a line, which showed unexpected segregation rate and gave us no double disruptants. Reciprocal crosses suggested that pollen from the line won't fertilize.
