Abstract
Leaves are indeterminate organs and possess genes involved in establishing leaf dorsoventrality. These polarities are established relatively early during leaf development and defined relative to the SAM. However, no direct factor has been found in the genetic interaction between meristem and differentiation of leaf organ. Our previous study revealed that. Deformed Root and Leaf1 (DRL1) is involved in the regulation of meristem activity and leaf defferentiation. The DRL1 was found to encode a novel protein showing homology to Elongator-associate protein (EAP) of yeast KTI12. The expression of DRL1 of Arabidopsis can complement KTI12, suggesting that DRL1 may act as an EAP in higher organism. To characterize the function of EAP, we identified the genes encoding RNAPII Elongator subunits (AtELPs) of Arabidopsis. The molecular architecture of Arabidopsis holo-Elongator has investigated by the use of yeast two-hybrid system. Based on these results, we will discuss the putative function of DRL1 and AtELPs.
