Abstract
B3 domain is the plant specific DNA binding domain. More than 40 genes were found to code proteins that contain B3 domain in Arabidopsis. B3 domain family contains ABI3, FUS3, LEC2 and ARFs, which have been shown to play an important role to the plant growth and developments, such as regulation of the expression of seed-storage genes or auxin signaling. However, the functions of most of the B3 genes remain to be clarified.
To analyze the function of B3 family genes, we applied CRES-T system to this family and expressed the chimeric repressor for B3 protein to suppresses the target gene in Arabidopsis. Some of the transgenic plants that expressed the chimeric repressor resulted in the fusion of cotyledon and absence of SAM of seedling. In addition the chimeric repressor affect leaf and flower organ formation, root elongation and embryogenesis. We will discuss the possible function of B3 domain proteins.
