Abstract
We have been analyzing the function of a barley protein (P23k) which express abundantly in scutellum of imbibed barley seed. Interestingly, the expression of P23k is also detected in aleuron layer and vascular bundles of ripening seed. These results suggest that P23k is involved in the function of seed. In this study, we examined the intercellular localization of P23k using cell fractionation experiment and immunofluorescence microscopy to know the function of P23k.
The cell fractionation and Western blot analysis revealed that the P23k fraction is nearly identical to the fraction of an endoplasmic reticulum (ER) marker enzyme, cytochrome c reductase. The P23k localization in ER was also confirmed by immunofluorescence microscopy study. These results indicated that P23k is a functional protein localized in ER. In this meeting, we will report the intercellular localization of P23k and discuss the function of P23k in seed.
