Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 47
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Functional Analysis of Methyl Chloride Synthase Genes in Arabidopsis.
*Yukari NagatoshiShinji IshiharaTatsuo Nakamura
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Pages 683

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Abstract
Methyl chloride (CH3Cl) contributes to the destruction of stratospheric ozone. Recent studies clarified that plants are the largest source of CH3Cl, and a methyl chloride synthase gene, designated as HOL, was identified in Arabidopsis. It could be predicted that HOL also methylates thiocyanate ion (NCS-). Although the postulated roles of the enzymes include conferring chloride tolerance or production of methyl thiocyanate, which has repellent activities, none of the direct evidences in vivo has been reported so far.
We isolated Arabidopsis T-DNA insertion mutants for HOL and its homologs, HOL-like1 and HOL-like2, and analyzed the responses of the mutants to probable substrates. As a result, the hol mutant solely showed an increased sensitivity to NCS-, suggesting that HOL is responsible for NCS- metabolism in vivo. Further analysis of the mutants, enzyme kinetics, environmental responsivity and tissue specificity of the gene expression are underway and will be presented.
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© 2006 by The Japanese Society of Plant Physiologists
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