Physiological functions of a rice putative voltage-gated Ca²⁺-permeable channel, OsTPC1, in defense signaling: transcriptomic analyses using retrotransposon-insertional functional knockout mutants.

Abstract

Elicitor-triggered transient membrane potential changes and Ca²⁺ influx through the plasma membrane are thought to be important during defense signaling in plants. However, the molecular bases for the Ca²⁺ influx and its regulation remain largely unknown. We identified a putative voltage-gated Ca²⁺-permeable channel (OsTPC1) in rice (Plant Cell Physiol. 2005 45: 693-), and developed its overexperssors as well as its retrotransposon-insertional functional knockout mutant (Ostpc1). OsTPC1 has been suggested to determine sensitivity to a proteinaceous elicitor and plays a role as a key regulator of elicitor-induced defense responses, activation of MAPK cascade and hypersensitive cell death. (Plant J. 2005 42: 798-809). Oligomicroarray transcriptome analyses revealed significance of OsTPC1 in regulation of pathogenic elicitor-induced defense gene expression.