Abstract
Suspension-cultured cells of Arabidopsis thaliana could be an excellent model system to study physiological/biochemical responses induced by PAMPs and compare with the results obtained from the molecular genetic studies with the use of various Arabidopsis thaliana mutants. We show here that the suspension-cultured Arabidopsis thaliana cells respond to various PAMPs, including bacterial LPSs, flg22 peptide, chitin oligosaccharides, but not to chitosan oligosaccharides and linear laminarioligosaccharides, for the induction of ROS generation. The ROS generation required protein phosphorylation but not protein synthesis. The involvement of PLC/PLD in this process was also indicated. These results were mostly similar to those obtained by suspension-cultured rice cells as well as intact A. thaliana seedlings but partly different. The results indicate the usefulness of the cell culture system for the detailed analysis of PAMPs recognition and responses.
