Abstract
OsGI is one of the genes involved in the photoperiodic control of flowering in rice, however its biochemical function is unknown. To understand that, we attempt to identify members in the OsGI protein complex under native conditions by using the TAP method. The TAP method showed the rapid purification of complex without prior knowledge of the complex composition, activity or function.
In order to isolate the OsGI complex, we produced TAP tag constructs, N-terminal TAP (SGN) and C-terminal TAP (SGC) and transformed them into rice. OsGI and OsGI-TAP proteins were detected by western blot analysis with OsGI specific antibody. OsGI was localized in cytosol and nucleus by the analysis of GFP fusion genes in transient assay. OsGI complex from the cultured cell (SGC lines) were isolated by using the TAP method. Identification of proteins included in the OsGI protein complex is being carried out by massspectrometric method.
