Abstract
To identify useful genes of rice, we generated rice FOX (full-length cDNA overexpressor) lines that rice full-length cDNAs were over-expressed in Arabidopsis plants. In this study, we have used imaging of chlorophyll fluorescence to screen photosynthesis-related mutants. Chlorophyll fluorescence was measured using rice FOX T2 generation. Time course of changes in chlorophyll fluorescence intensity and photosynthetic parameters were compared with WT. So far, we isolated several types of candidates with altered photosynthetic parameters. To isolate high-light tolerant mutants, chlorophyll fluorescence was also measured after high-light treatment (2,000 µE, 1 h). In addition to the chlorophyll fluorescence measurement, leaf temperature was monitored using thermal imaging system to identify mutants with altered transpiration. We will report on the results of these screenings on this meeting. This research is supported by the Special coordination funds for Promoting Science and Technology entitled Rapid identification of useful traits using rice full-length cDNAs.
