Evaluation of Arabidopsis FOX line:T2 phenotype and phenotype recapitulation by transformation.

Abstract

We describe here the establishment of a novel gain of function system named as Fox hunting system (Full-length cDNA over-expresser gene-hunting system) for random over-expression of a normalized Arabidopsis full-length cDNA library. Full-length cDNAs from Arabidopsis were sequenced and c.a. 10,000 non-redundant clones were randomly expressed in Arabidopsis plants. We observed 1,487 morphological mutant candidates while growing 15,547 T1 transformants. One of the most frequently observed phenotypes was that of pale green leaves and stems. When 115 such T2 lines were observed 59 of these lines showed the pale green phenotype in dominant or semi-dominant fashion. We will introduce 10 different lines that showed the visible phenotypes upon retransformation of the wild type plant with the rescued full-length cDNAs. Applications of this system on rice functional genomics will be discussed.