Abstract
FOX hunting system is novel gain-of-function technique. In this system, random over-expression of a normalized plant's full-length cDNA library cause dominant gain-of-function mutations and it is expected that plant acquire useful phenotype. We are establishing rice FOX lines using 13,000 non-redundant rice full-length cDNAs to introduce them into Arabidopsis plants. At present, we are screening these lines with various trait for exploration of useful rice genes. In this study, we will introduce screening strategy for isolation of mutants of higher accumulated elements and pigments (Elements and Pigments) in rosette leaves as examples of useful phenotype. We already isolated some mutants by pre-screening in T1 generation from about 3,000 lines.
This research is supported by the Special coordination funds for Promoting Science and Technology entitled Rapid identification of useful traits using rice full-length cDNAs.
