Abstract
The Golgi apparatus plays a key role in sorting and delivering proteins of various destinations. The Golgi apparatus possesses very unique architecture, which varies depending on the species. However, the relationship between the function and the structure of the Golgi apparatus is not clear, and how the Golgi apparatus maintains its unique architecture is left almost unknown. We are studying the mechanisms of the biogenesis of Golgi apparatus using higher plant, Arabidopsis, as a model system. First, we observed detailed structure and dynamic movement of the Golgi apparatus in living cells under a high-speed and high-sensitivity confocal laser scanning microscope. We are also screening mutant Arabidopsis showing abnormal Golgi morphology. We will report interesting phenotypes of putative mutants as well as the effect of the loss of function of some SNAREs on Golgi.
