Abstract
The TGN is one of the most important organelles for protein transport at the post-Golgi network, and functions as a sorting spot that directs cargo proteins to a variety of post-Golgi compartments. However, the TGN of plant cells has not been well understood yet. In order to elucidate the structure, function and dynamics of plant TGN, we focused on AtTLg2a/Syp41, the ortholog of Tlg2/syntaxin16 which is localized to the TGN in
yeast and mammalian cells, as a TGN marker. We established the transgenic plants expressing GFP-Syp41/AtTlg2a under the control of the native promoter. The observation under the CLSM (confocal laser scanning microscopy) showed that the TGN is a dot-like BFA (brefeldin A)-sensitive and Wm (wortmannin)-insensitive compartment, which is partially stained with FM4-64. These results suggest that the TGN functions in endocytic pathways in addition to the biosynthetic pathways. We will also discuss dynamics between TGN and the Golgi apparatus.
