Abstract
We have isolated a novel gene, OsPI1 from P-deficient rice. Low P induces OsPI1 immediately, therefore, it is suggested that OsPI1 plays an important role in low P adaptation. OsPI1 is thought to belong to TPSI1/Mt4 family, which contains low P inducible non-coding RNAs. Though it is suggested that an Arabidopsis gene of this family related to phosphate-recycling, the detail is still unclear. The purpose of this study is to reveal the function of OsPI1 using OsPI1 knockdown lines.
Seedlings of wild type (WT) and 2 knockdown lines (KD) were hydroponically cultured for 10 d with or without P and used for microarray analysis. Alterations of expression of several genes involved in phosphate-recycling under P-deficiency were smaller in KD than in WT. Furthermore, total P concentration and root/shoot of P-deficient KD was higher and lower than P-deficient WT, respectively. These results suggest that OsPI1 has a critical role in phosphate-recycling.
