Abstract
The resource of phosphate rock has been depleting, whereas a large amount of organic-P (Po) exists as unavailable form in soil. To liberate inorganic-P (Pi) from Po, plants secret acid phosphatase into the rhizosphere. White lupin has high ability to secret acid phosphatase and grows well under P deficient conditions.
A gene encoding secreted acid phosphatase had been isolated from white lupin and transformed to tobacco plants. The aim of this study is to investigate the ability of P-acquisition by LASAP2 transgenic tobacco. P uptake and growth of LASAP2 line were higher than wild type in sterile medium containing phytate as sole P source. When plants were grown in pots containing soils of long-term experimental field, growth and P accumulation of LASAP2 line were higher than wild type in -P and phytate treatments. These results suggest that overexpression of LASAP2 is effective for P-acquisition from soil Po, including phytate.
