Abstract
So many Al stress tolerance mechanisms for plants have been proposed, but we believe that characterization of the response mechanism is also very important to solve this problem. In this study, we tried to isolate the genes encoding transcription factors related to the gene-expression of the Al inducible Arabidopsis gene, AtGST11, using our unique screening system, bio-panning.
A cDNA library was constructed using T7 phage and mRNA derived from Al treated Arabidopsis and repeatedly screened three times by a DNA (promoter region of AtGST11; p-AtGST11)-protein binding reaction (bio-panning). DNA sequencing indicated that they were an unknown protein, a RING finger protein and HD-Leucine zipper protein. A gel-shift assay using p-AtGST11 and these purified proteins indicated that they can bind to the region of -200 to 0 from ATG site in p-AtGST11 and suggested that they may function for the expression of AtGST11 under Al stress.
