Plant Metabolic Profiling in Combination with Statistical Analysis of Metabolite Changes and Metabolite-metabolite Correlation Analysis for Arabidopsis

Abstract

In the former annual meeting held on 2005, we introduced the metabolome pipeline by using GC-TOF/MS. Here we show the application of plant metabolic profiling by using our developed pipeline.
The aerial parts and roots of wild-type (Col-0) and well-studied mutants (mto1-1 and tt4) were harvested, and subsequently the obtained samples were extracted, derivatized and then analyzed by using our GC-TOF/MS-based metabolic profiling pipeline. Finally, we could acquire 163 annotated peaks (96 peaks as known metabolites) in aerial parts and 166 annotated peaks (97 peaks as known metabolites) in roots, respectively. The behaviors of metabolites detected could be represented in combination with statistical analysis and correlation analysis. The pattern of metabolite accumulation observed in aerial parts of mto1-1 was largely consistent with the findings presented in the previous publications. For tt4, the unique metabolite-metabolite relationships were observed from the result of metabolite-metabolite correlation analysis when compared with those of Col-0.