Abstract
Plant isoprenoid pathways produce various metabolites including important industrial materials such as rubber. To investigate regulatory mechanisms of the pathways, we analyzed co-expression networks with correlation coefficients that were calculated from the 771 microarray data of AtGenExpress. According to the outcome networks, we selected 12 genes as candidates for further analysis. Full-length cDNA (RAFL) clones for the genes were obtained from RIKEN and overexpressed them under 35S CaMV promoter in the T87 Arabidopsis suspension-cultured cells. The transgenic cell lines were analyzed with DNA microarray and non-targeted metabolite profiling using GC/MS, UPLC-Q-TOF/MS and FT-ICR/MS. We preliminarily found that two candidate genes actually affected gene expression of MEP and MVA pathway enzymes, and that several unidentified peaks in GC/MS distinguished the overexpression lines from control. Currently we are working on identifying the metabolite peaks influenced as well as analyzing transgenic cell lines for the other candidate genes.
