Expression of tobacco purple acid phosphatase in Arabidopsis thaliana

Abstract

We suggest that purple acid phosphatases (PAPs) have a physiological role relating to β-glucan deposition in suspension-cultured tobacco cells. To study the function of PAP in an intact plant, we expressed tobacco PAP (NtPAP12) constitutively in Arabidopsisi thaliana. In this work, we analyzed the phenotypes between the transgenic lines, T-DNA knock-out lines of AtPAP10 and the wild-type. Since AtPAP10 has the highest homology to NtPAP12 among Arabidopsis PAP genes, we suppose that roles of AtPAP10 correspond to NtPAP12. The cells of rosette leaves in the transgenic lines were smaller in diameter than those in the wild-type and the knock-out line. We will analyze compositions and quantities of wall polysaccharides in the three lines.