Abstract
The cell wall is important for maintaining cell shape and rigidity. Although the structural framework of the cell wall consists largely polysaccharides, proteins may also participated in networks of the wall. In order to understand the function of the cell wall proteins, we identified dark-grown hypocotyl of black gram (Vigna mungo L.) cell wall proteins. The cell wall proteins were extracted with 3 M LiCl (pH 6.8) and applied to two-dimensional gel electrophoresis. Five protein spots were analyzed for NH2-terminal amino acid residues and 10 amino acid residues were determined. Based on the residues information, we have cloned and sequenced the genes of two of them (P1 and P5). P1 showed homology with unknown protein of Medicago sativa and P5 with curculin-like (mannose-binding) lectin of Medicago truncatula. The functional roles of P1 and P2 in wall rigidity or cell elongation are made investigation.
