Abstract
F-box protein acts as a receptor for target protein of ubiquitination in SCF complex. By database analysis, we found 568 F-box protein genes in Arabidopsis genome, however functions of most of the F-box proteins are still unclear. To carry out large-scale functional analysis we have cloned ORFs of F-box protein genes in recombination type cloning vector. We first employed RT-PCR cloning using total RNAs from various tissues to clone novel splicing variant as well as predicted ORFs. As to ORFs that was not cloned by the RT-PCR, we amplified the ORFs using the full-length cDNAs. All cloned PCR fragment were sequenced. This cloning resulted in finding lots of unpredicted transcripts that were candidates for splicing variants. We characterized the splicing variants by the analysis of their sequences, structures and expression patterns. We present here the results of the ORF cloning, characterization and expression study of cloned ORFs.
