Abstract
In order to investigate the whole genetic system of legume species, we initiated a large-scale genome analysis of a model legume, Lotus japonicus.
Combining clone-by-clone sequencing of large insert clones from gene-rich regions with random genome sequencing, genome sequence information covering approximately 90% of the L. japonicus ESTs has been accumulated. By generating PCR-based markers from the accumulated genome sequences, positional information on 80 % of the sequenced clones has been obtained. The sequence data, predicted gene information and mapping information are available through the web database, http://www.kazusa.or.jp/lotus/. In order to establish the basis for transfer of knowledge on L. japonicus to other legumes, comparative genome analysis between L. japonicus and other legumes is currently in progress.
The latest status of the genome sequencing project and the features of the L. japonicus genome appearing from the functional annotation and comparative genome analysis will be presented.
