Abstract
We studied the effect of glucose on amino acid transport of symbiont from Japanese Paramecium bursaria. Algal Ser uptake was increased by glucose even at its low concentration (EC50=3 μM), and radiolabeled glucose uptake was not detectable. From these results, we considered that the acceleration by glucose occurred via signaling pathway. Uptakes of Ala and Gln, which were transported via general amino acid transport system, were also accelerated, indicating the association of the transporter and the response. In kinetic analysis of Ser uptake, 2-fold Vmax value was measured in the presence of glucose. When the protein synthesis of cells was inhibited, these cells also showed the response which was not due to new protein synthesis. The results using glucose related compounds indicated that an importance of stereochemistry at carbon 1, 2 and 5, and hydroxyl group at carbon 3 and 6 for the response.
