Isolation and characterization of sugar insensitive mutant in Arabidopsis

Abstract

Sugars act as not only source of energy and carbon skeletons, but as signaling molecules to regulate multiple biological processes in plants.
To clarify mechanisms involved in sugar response, FOX (full length cDNA over-expressor) hunting system was used to isolate gain-of-function mutants for glucose insensitivity. Screening on the selection medium that contains highly concentrated glucose (300 mM) and lowly concentrated nitrogen (0.1mM) succeeded in isolation of the glucose insensitive mutant, named ssv1 (super survival 1). The ssv1 mutant shows greening and developing true leaves on the selection medium, while wild type plant shows white cotyledons and terminates leaf development leading to die. The mutant is due to overexpression of the SSV1 gene, which encodes Ring finger type E3 ubiquitin ligase. Detailed characterizations of the mutant will be reported.