Abstract
To identify natural variability in salt stress tolerance among Arabidopsis thaliana ecotypes, we compared the salinity tolerance among 354 A. thaliana ecotypes collected in RIKEN BRC. We obtained some salt tolerant or sensitive ecotypes compared with standard strain, Col-0. As for a tolerant ecotype, Bu-5, it showed salt tolerance clearly compared with Col-0 in consequence of salt acclimation with 100 mM NaCl treatment which does not affect the growth. To determine whether the salt acclimated Bu-5 is tolerant to osmotic stress or to specific ions, the salt acclimated seedlings were treated with LiCl, CsCl, or sorbitol. As a result, the salt acclimated Bu-5 seedlings were also tolerant to sorbitol, but not to LiCl and CsCl.
We are carrying out microarray analysis to identify transcriptional changes during salt acclimation between Bu-5 and Col-0 seedlings. Furthermore, we mapped the gene responsible for the salt tolerance of Bu-5 in 100kbp on chromosome 5.
