Abstract
In Arabidopsis thaliana, leaves have adaxial side (upper side) and abaxial side (lower side), which differ in shapes of cells and in the number of stomatas or trichomes. In order to examine genetic mechanism determining the adaxial/abaxial boundary in leaf primodia, we have isolated new mutants in which the expression pattern of the abaxial specific gene, FIL, is abnormal. One mutant, #1-63, formed abaxializing or adaxialing leaves. Expression domein of PHB, adaxial marker, and FIL were also changed. These results indicate that #1-63 was defective in the determination of adaxial-abaxial boundary site. We will report analyses of phenotypes and expression patterns of adaxial/abaxial makers in #1-63. Cloning of the gene is in progress.