Abstract
In plant lateral organs, including leaves, floral organs and flowers, tissues characteristic of different regions (i.e. adaxial, abaxial and lateral regions) are developed in the axis-dependent manner. In contrast to adaxial and abaxial regions, it is not well understood how lateral region is specified at the molecular level.
PRS was identified to be necessary to form the lateral-specific tissues, and specifically expressed in the region (Matsumoto and Okada, 2001).
For further investigation of factors involved in lateral specification, we examined deletion series of the PRS promoter to identify cis elements. A 140-bp region, named region A, is identified to be necessary for the lateral-specific expression. This region A was also sufficient for the expression in the early stages of lateral-organ development. Now we are screening for trans-acting factors binding to the region A using yeast one-hybrid system.
