Abstract
It has been reported that mature dry seeds contain RNAs stored during maturation and protein synthesis in the early stage of seed germination occurs utilizing such long-lived RNAs. However, the mechanism for keeping the activities of long-lived RNAs and the induction system of protein synthesis after imbibition remains to be elucidated. There is a possibility that RNA binding proteins in dry seeds play important roles in stabilization of long-lived RNAs and/or regulation of protein synthesis. It is well known that RNA binding proteins could be purified selectively by single stranded DNA (ssDNA)-affinity column chromatography. Therefore, we carried out proteome analysis of proteins in the ssDNA binding fraction from dry seeds of rice. It was found that RNA binding proteins such as glycine rich RNA binding protein and KH domain protein were detected in the fraction. In addition, the KH domain containing protein was disappeared until 24 hr after imbibition.
