Abstract
CRES-T is a dominant and efficient gene silencing system which represses the expression of target gene and is consequently effective even in the presence of functionally-redundant transcription factors. We evaluate CRES-T as a tool for the modification of phenotypes through the production of transgenic Pharbitis nil. In AP3SRDX flowers, changes in phenotype was not observed. In AGSRDX flowers, development of sepals, petals and stamens were suppressed and pistil protruded from sepals in early stage, showing not loss-of-C-class function phenotypes but loss-of-B-class function. Results of RT-PCR analysis showed that changes in expression patterns of endogenous B function genes, namely PnAP3 and PnPI. These results suggest that CRES-T system functions in Pharbitis nil, while there are some variations in function of transcription factors among plant species.
This study is supported by the Research Project for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries.
