Abstract
Previously, we isolated a novel Zinnia peroxidase gene, ZPO-C, using Zinnia xylogenic culture system. ZPO-C was suggested to be involved in lignification of tracheary elements. In this study, we performed promoter-GUS assay to investigate the function of ZPO-C-type peroxidases in planta. We generated transgenic Arabidopsis lines carrying AtPrx66 (Arabidopsis homolog of ZPO-C), AtPrx47 and AtPrx64 (peroxidases showing highest homology to AtPrx66 among 73 Arabidopsis peroxidase genes) promoter-GUS reporter gene fusions. Histochemical GUS analysis using seedlings showed that although GUS signals were mainly localized in roots, their patterns of localization were quite different among these promoters. The GUS signal was detected in developing vessels in AtPrx66, in vessels and steles in AtPrx47, and in outer regions of steles in AtPrx64. In addition to these results, we will report GUS expression patterns in adult plants, and discuss the roles of these peroxidases in planta.
