In Vitro Chloroplastic Protein Import Experiments with Recombinant Precursor Proteins

Abstract

We have developed the in vitro chloroplastic protein import system free from the radio-labeled proteins with use of recombinant precursors. The import efficiency of this system is improved from the previous reports, thus we are able to handle the newly imported protein in chemical amounts. We are currently developing the application of this in vitro chloroplastic protein import system. We will present our current effort on the development of the application.