Abstract
In the vesicle transport systems of eukaryotes, COPII that consists of the small GTP-binding protein, Sar1p and two coat protein complexes, sec23p/24p complex and sec13p/31p complex, work in the transport from ER to golgi apparatus. In this research, we examine the function of COPII components in development of Arabidopsis thaliana.
In these COPII components, we carry out the analysis of ATSEC31s, ATSEC13s, ATSEC23s and ATSEC24s. The homology search indicates that there are two homologs for Sec31p (ATSEC31), two homologs for Sec13p (ATSEC13), seven homologs for Sec23p (ATSEC23) and three homologs for Sec24p (ATSEC24). In this research, we carry out the expression analysis and the intracellular localization analysis. In this poster, we report about the intracellular locatization analysis for ATSEC31, ATSEC13 and ATSEC23. Additionally, we examin whether ATSEC31 and ATSEC13 interact each other. In this examination, the fluorescence of YFP was detected, suggest that ATSEC31 interacts with ATSEC13.
