Abstract
In pollen, the membrane structure is largely reconstructed throughout the pollen development and pollen tube elongation. Particularly, large amount of cell wall and plasma membrane (PM) components are selectively transported to the tip region of pollen tube during the pollen tube elongation process. In order to search SNARE (soluble N-ethyl-maleimide sensitive factor attachment protein receptor) molecules which are involved in the pollen maturation or pollen tube elongation, we generated transgenic Arabidopsis expressing GFP-fused PM Qa-SNARE molecules under the control of their own promoters, and found that totally four SNARE molecules (SYP112, SYP124, SYP125 and SYP131) were specifically expressed in the pollen. We also report the subcellular localization of these SNAREs in pollen.
