Abstract
Phytoremediation is a good technique to clean or remediate Cd-polluted soils. In order to practicalize this technique, it is essential to control long-distance transport of Cd in vascular tissue. In this study, we tried to investigate proteins in the phloem sap to elucidate the mechanism of long-distance Cd transport in the sieve tubes.
Oilseed rape plants (Brassica napus L.) were grown hydroponically for about three months and treated with 30μM Cd for 48 hours. Phloem sap was collected from these plants after Cd treatment. Effects of Cd on sugars and amino acids concentration in the phloem sap were not observed. Additionally, difference was not found in protein concentration in the sap (protein concentration in the phloem sap was approximately 0.25 mg mL-1). 2D-PAGE analysis revealed that several proteins in the phloem sap responded to Cd treatment. We tried to identify these proteins by PMF analysis.
