Abstract
Spores of Osmunda japonica are suitable for the material of biochemical studies, because it is possible to prepare plentiful spores. However, we have some experience getting the spore preparation with low viability. If the viability of spores depends on the humidity at the time of spore preparation, this phenomenon has been verified in this study. Spores were treated by various relative humidity (RH) for 24 hours, and then used to determine the viability. The viability of the spores treated by 0.3% RH was very low. The viability became larger as RH increases from 0.3% RH to 60% RH and fell off to the bottom at 81% RH. It was elevated again to the largest viability by increasing toward 100% RH. Clearly the difference of humidity in the time that prepared spores led to the dispersion of viability. The transfer speed of water may relate to the viability of spores.
