Abstract
ABA plays important roles in plant development and responses to various environmental stresses. ABA is synthesized in response to drought, and ABA promotes the closing of opened stomata and inhibits the opening of closed stomata. The resulting stomatal closure reduces transpirational water loss and maintain water homeostasis in the plants. Proteomics will be extremely useful approach to investigate signal transduction pathway, because protein-protein interaction and post-translation modification including phosphorylation often plays a major role in determining the final effectiveness of signal transduction pathways. The crude protein mixtures were prepared from epidermal tissues of Arabidopsis thaliana Col-0.
They were subjected to 2D-PAGE. The gels were stained with Pro-Q Diamond for detecting phosphorylated proteins and with CBB for all proteins. The spots were digested with lysyl endopeptidase and characterized by MALDI-TOF MS. Several proteins were up regulated in response to ABA treatment (50uM for 5 min).
