Abstract
The plant activator is very useful for plant disease control and management. However, development of the plant activator is very difficult and challenging because it has no toxic effect against fungi and bacteria and works only through the plant. We tried development of screening systems for plant activator using plant immune system. We developed a reporter gene-assay system using promoters of the defense-related genes. To validate the strategy, we performed subsequent analysis using an Arabidopsis microarray consisting of 1,200 full-length cDNA clones representing putative defense-related and regulatory genes. Furthermore, we prepared a B. rapa cDNA microarray using ca. 2 K cDNA clones selected from 2,166 non-redundant sequences of cDNA library of Chinese cabbage. We applied B. rapa microarray to evaluate the performance of Arabidopsis microarray for the screening of plant activators. In this report, we will suggest that this novel system can be used to screen for candidate plant activators.
