Abstract
The NMR-based metabomics approach has much potential for not only basic science but also for applied science in plant systems. Firstly, 1D-1H-NMR metabolic fingerprinting can be used for characterization of global metabolic changes such as RIKEN Arabidopsis disruptome collection and core-collections of rice and wheat strains. Identification of changed metabolites would be performed by multi-dimensional NMR coupled with stable isotope labeling of plants and by use of our original Java software SpinAssign and original standard database. In addition to this, the 13C labeling can be used in metabolic flux analysis of plant cells at atomic level. Furthermore, we have observed nicely resolved 1H-13C cross peaks of non-extracted, insoluble small and macromolecules by magic-angle-spinning NMR experiments. Finally, the 13C-labeled plant biomass can be also used as nutritional resources for animal systems. We will show how the 13C labeled plants can be metabolized by animal organs using the NMR technologies.
