Abstract
[Background] In spinach, integrated relationship among metabolites and nitrogen (N) nutrition is still ambiguous. We examine the influence of different nitrogen source under hydroponics conditions on spinach and focused to the profile of metabolite by using GC-MS. [Method] In Experiment 1, N concentration of nutrient solution was supplied by NO3 at the rate of 1, 2 and 4 mmol L-1, respectively. In Experiment 2, NO3 and NH4 were applied with the ratio of 10:0, 5:5 and 3:7, respectively. The extraction media (Methanol: Chloroform: Water =33: 16: 23 ) was added to lyophilized leaf disks. After TMS derivatization, each sample was injected into GC-MS. [Results] Principal component analysis shows that components reflected the amount of nitrogen amount and form applied. From the loading analysis, amino acids correspond to the nitrogen amount and form, and sugars and organic acids correspond to the amount of nitrate applied.
