Identification and characterization of transcription factors regulating methionine-derived glucosinolate biosynthesis I

Abstract

Integration of transcriptomics and metabolomics enables us to predict the co-regulated genes and metabolites in Arabidopsis. This strategy is useful to identify a set of genes involved in the same metabolic pathway and its transcription factors [1]. In this study, we analyzed the transcripts and metabolites in knockout and over-expression lines of PMG1, PMG2 and PMG3, transcription factors of methionine-derived glucosinolate (MET-GSL) biosynthesis genes, which were predicted by coexpression analysis [1]. In leaves and seeds of a double knockout line pmg1pmg2, MET-GSLs were not detected, and the expression of MET-GSL biosynthesis genes and PMG3 were remarkably repressed in leaves. In seeds of a PMG2 over-expression line, MET-GSL accumulation increased by 10-fold. These results suggest that PMG genes are major transcription factors regulating the MET-GSL biosynthesis genes. [1] PNAS 104, 6478-6483